Re-crystallization in 70% MeCN/30% water.
1.Heat a saturated solution of the matrix in 70% MeCN/30% water until boiling.
2.Carefully boil until solid dissolves completely.
3.Cool to room temp, then on ice – precipitate should form.
Protocol for re-crystallization of alpha-cyano-4-hyrdoxy-cinnamic acid
1.To 100mg of alpha-cyano-4-hydroxy-cinnamic acid, add 10ml of water
2.Add ammonium hydroxide until most of acid dissolves.
3.Slowly add concentrated HCl to the solution until a large amount of the acid has precipitated (about pH 2).
4.Remove the precipitate by centrifugation or filtering.
5.Wash the precipitate several times with 0.1M HCl.
6.Dry matrix and store at -20 C in the dark.