Advancing Patient Care Through Research
Washington University School of Medicine

Peptide Mass Calibration Standards

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Name Source Monoisotopic Mass [M+H]+ Avg Mass [M+H]+ Sigma Swiss-Prot Reference
Leu enkephalin Human 556.277 556.6 L9133 PENK_HUMAN
Met enkephalin Human 574.234 574.7 M6638 PENK_HUMAN
[D-Ala2]-Deltorphin II 782.396 782.9 DAD2_PHYBI
Angiotensin II Human 1046.542 1047.2 A9535 ANGT_HUMAN
Bradykinin Human 1060.5692 1061.23 B3259 KNH_HUMAN
Lutenizing hormone
releasing hormone
Human 1182.581 1183.3 L7134 GONL_HUMAN
Ala-Pro-Gly-[Ile3,Val5]
Angiotensin II
Human 1271.654 1272.5 A0289 ANGT_HUMAN
Angiotensin I Human 1296.6853 1297.50 A9650 ANGT_HUMAN
Substance P 1347.7360 1348.7 S6883 TKNB_HUMAN
α-endorphin Human 1745.842 1747.0 E6136 COLI_HUMAN
Peptide sequencing standard 1657.841 1658.8 P2046
Neurotensin 1690.928 1692.0 N6383 NEUT_HUMAN
Renin substrate Porcine 1758.933 1760.1 R8129 ANGT_PIG
Renin substrate Human 1759.940 1761.0 R5880 ANGT_HUMAN
dynorphin 2147.1988 2148.52
Adreno-cortico-tropic
hormone fragment 18-39
Human 2465.199 2466.7 A0673 COLI_HUMAN
Insulin A chain, oxidized Bovine 2530.921 2532.7 I1633 INS_BOVIN
Melittin Bee Venom 2845.762 2847.5 M1407 MEL1_APIME
β-endorphin 3463.8296 3466.04
Glucagon Bovine 3481.624 3483.8 G7774 GLUC_BOVIN
Insulin B chain, oxidized Bovine 3494.651 3496.9 I6383 INS_BOVIN
Adreno-cortico-tropic
hormone fragment 7-38
3657.929 3660.2 A1527 COLI_HUMAN
Insulin Sheep 5700.598 5704.5 I9254 INS_SHEEP
Insulin Bovine 5730.609 5734.6 I5500 INS_BOVIN
Insulin Equine 5744.624 5748.6 INS_HORSE
Insulin Porcine 5774.635 5778.6 INS_PIG
Insulin Human
(Recom-binant
in E. coli)
5804.646 5808.6 I0259 INS_HUMAN
Ubiquitin Bovine 8560.625 8565.8 U6253 UBIQ_BOVIN